Genome wide association studies (GWAS) of element contents in grain with a special focus on zinc and iron in a world collection of barley (Hordeum vulgare L.)

Published Date
September 30, 2017
Type
Journal Article
Genome wide association studies (GWAS) of element contents in grain with a special focus on zinc and iron in a world collection of barley (Hordeum vulgare L.)
Authors:
Sanjaya Gyawali
Marinus L. Otte, Shiaoman Chao, Abderazzek Jilal, Donna L. Jacob, Amezrou Reda, Ramesh Pal Singh Verma

Genome wide association studies (GWAS) were carried out to map Quantitative Trait Loci (QTL) associated
with element contents in the grain using 336 spring barley. Of the elements analyzed, Fe content
ranged from 21.9 to 91.0 mg kg1, Zn from 10.4 to 54.5 mg kg1, Ba from 0.2 to 8.9, Ca from 186.4 to 977.5,
Cu from 1.5 to 9.8, K from 353.2 to 7721.5, Mg from 1049.8 to 2024.2, Mn from 8.1 to 22.9, Na from 55.9 to
627.9, P from 2272.9 to 5428.8, S from 880.7 to 1898.0, Si from 19.1 to 663.2, and Sr from 0.35 to
2.62 mg kg1. GWAS were carried out using 6519 SNP markers and multiple elements in MLM:PCA þ K
model in TASSEL software. Population analyses showed two sub-populations, primarily based on row
types. GWAS for row types showed association with INTERMEDIUM-C, a modifier gene for lateral spikelet
fertility in the 4H chromosome, validating current GWAS approach. GWAS also showed that 2 QTL for Ba,
2 for Ca, 4 for Cu, 11 for Fe, 2 for K, 3 for Mg, 6 for Mn, 4 for Na, 3 for S, 5 for Si, and 3 for Zn were mapped
in barley chromosomes. The QTL identified in the current study are valuable for breeding nutrient dense
barley cultivars in the future, especially Zn and Fe.

Citation:
Sanjaya Gyawali, Marinus Otte, Shiaoman Chao, Abderazzek Jilal, Donna Jacob, Amezrou Reda, Ramesh Verma. (30/9/2017). Genome wide association studies (GWAS) of element contents in grain with a special focus on zinc and iron in a world collection of barley (Hordeum vulgare L. ). Journal of Cereal Science, 77, pp. 266-274.