Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

Published Date
April 28, 2006
Type
Journal Article
Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance
Authors:
Imad A. Eujayl
William Erskine, Bassam Bayaa, Michael Baum, E. Pehu

The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.

Citation:
Imad A. Eujayl, William Erskine, Bassam Bayaa, Michael Baum, E. Pehu. (28/4/2006). Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance. Plant Breeding, 177 (5), pp. 497-499.
Keywords:
resistance
rapd markers
recombinant inbred lines
fusarium oxysporum f. sp lentis
lens culinaris