Genetic Dissection of Drought Tolerance of Elite Bread Wheat (Triticum aestivum L.) Genotypes Using Genome Wide Association Study in Morocco

Published Date
October 13, 2022
Type
Journal Article
Genetic Dissection of Drought Tolerance of Elite Bread Wheat (Triticum aestivum L.) Genotypes Using Genome Wide Association Study in Morocco
Authors:
EL Gataa Zakaria
Samir Karima, Wuletaw Tadesse

Drought is one of the most important yield-limiting factors in Morocco. Identification
and deployment of drought-tolerant wheat varieties are important to cope with the challenge of
terminal moisture stress and increase wheat productivity. A panel composed of 200 elite spring
bread wheat genotypes was phenotyped for yield and agronomic traits for 2 years (2020 and 2021)
in Morocco under rainfed and irrigated environments. The panel was genotyped using 20K SNPs
and, after filtration, a total of 15,735 SNP markers were used for a genome-wide association study
(GWAS) using a mixed linear model (MLM) to identify marker-trait associations (MTA) and putative
genes associated with grain yield and yield-related traits under rainfed and irrigated conditions.
Significant differences were observed among the elite genotypes for grain yield and yield-related
traits. Grain yield performance ranged from 0.97 to 6.16 t/ha under rainfed conditions at Sidi
Al-Aidi station and from 3.31 to 9.38 t/h under irrigated conditions at Sidi Al-Aidi station, while
Grain yield at Merchouch station ranged from 2.32 to 6.16 t/h under rainfed condition. A total of
159 MTAs (p < 0.001) and 46 genes were discovered, with 67 MTAs recorded under rainfed conditions
and 37 MTAs recorded under irrigated conditions at the Sidi Al-Aidi station, while 55 MTAs were
recorded under rainfed conditions at Merchouch station. The marker ‘BobWhite_c2988_493’ on
chromosome 2B was significantly correlated with grain yield under rainfed conditions. Under
irrigated conditions, the marker ‘AX-94653560’ on chromosome 2D was significantly correlated with
grain yield at Sidi Al-Aidi station. The maker ‘RAC875_c17918_321’ located on chromosome 4A,
associated with grain yield was linked with the gene TraesCS4A02G322700, which encodes for F-box
domain-containing protein. The markers and candidate genes discovered in this study should be
further validated for their potential use in marker-assisted selection to generate high-yielding wheat
genotypes with drought tolerance

Citation:
EL Gataa Zakaria, Samir Karima, Wuletaw Tadesse. (13/10/2022). Genetic Dissection of Drought Tolerance of Elite Bread Wheat (Triticum aestivum L. ) Genotypes Using Genome Wide Association Study in Morocco. Plants, 11 (20).
Keywords:
gwas
wheat
mta
drought stress