A SSR kit to study genetic diversity in chickpea (Cicer arietinum L.)

Published Date
July 12, 2014
Type
Journal Article
A SSR kit to study genetic diversity in chickpea (Cicer arietinum L.)
Authors:
Rajeev Varshney
Mahendar Thudi, Hari D. Upadhyaya, Sangam Dwivedi, Sripada M. Udupa, Bonnie Furman, Michael Baum, David Hoisington

A chickpea simple sequence repeat (SSR) marker reference kit has been developed based on the
genotyping of the global chickpea composite collection (3,000 accessions) with 35 SSR markers.
The kit consists of three pools of chickpea accessions along with supporting documentation on
the SSR markers, polymerase chain reaction and detection conditions, and the expected allele
sizes for each of the 35 SSR loci. These markers were selected based on quality criteria,
genome coverage and locus-specific information content. Other important SSR selection criteria
were quality of amplification products, locus complexity, polymorphism information content
and well-dispersed location on a chickpea genetic map. The developed SSR kit has a wide
range of applications, especially for genetic diversity studies in chickpea. Using the markers
and reference accessions in the kit, scientists in other laboratories will be able to compare
the genotypic data that they obtain for their germplasm with that obtained using the global
composite collection.

Citation:
Rajeev Varshney, Thudi Mahendar, Hari D. Upadhyaya, Sangam Dwivedi, Sripada M. Udupa, Bonnie Furman, Michael Baum, David Hoisington. (12/7/2014). A SSR kit to study genetic diversity in chickpea (Cicer arietinum L. ). Plant Genetic Resources, 12(S1), pp. 118-120.